Effects of green tea and bisphosphonate association on dental socket repair of rats

Publication date: March 2017
Source:Archives of Oral Biology, Volume 75
Author(s): Edson Yoshihiro Mada, Alana Claro Cunha Santos, Angelica Cristina Fonseca, Claudia Cristina Biguetti, Fernando Tozze Alves Neves, Patrícia Pinto Saraiva, Mariza Akemi Matsumoto
ObjectivesTo evaluate the effects of green tea intake and zoledronic acid intravenous therapy on teeth socket repair.DesignSixty male albinus Wistar rats were divided into 4 groups: C—Control, intravenous (IV) 0.9% saline solution (SS), GT—1% green tea in drinking water and IV SS, BP—IV zoledronic acid (BP), and BP+GT—IV BP and 1% green tea. 0.035mg/kg of BP was administered every two weeks. After ten weeks, right upper molars were extracted and the green tea started to be offered for GT and BP+GT. After 7, 14, and 28days the animals were euthanized.ResultsHistopathology analysis revealed lack of socket repair in BP and BP+GT groups, which presented significant increased number of polimorphonuclear leukocytes at day 28, in comparison with C (p<0.05). No significant differences were detected between C and the experimental groups at the same period (p<0.05) when considering mononuclear leukocytes. Immunolabeling revealed that the association of BP and GT caused a slight disturbance in OPG/RANKL system and retarded Runx-2 labeling. Although strong TRAP labeling was observed, most of the positive cells in BP and BP+GT groups were not located on bone surface.ConclusionsSocket healing of rats treated with BP and regular drinking green tea presented no relevant differences in comparison to those treated with BP alone.



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