Abstract Infrared spectroscopy of cells and tissues is prone to Mie scattering distortions which grossly obscure the relevant chemical signals. The state of the art Mie Extinction Extended Multiplicative Signal Correction (ME‐EMSC) algorithm is a powerful tool for the recovery of pure absorbance spectra from highly scatter‐distorted spectra1. However, the algorithm is computationally expensive and the correction of large infrared imaging datasets requires weeks of computations. In this paper we...
Tue Aug 25, 2020 21:05
Abstract Exposure to ionizing radiation significantly affects biochemistry of cancer cells. The effect of irradiation can be divided into two stages, i.e. the physicochemical stage and the biological response. Both effects induce different biochemical changes in the cells and should be analyzed as two separate phenomena. Thus, in the current study, Raman spectroscopy of prostate cancer cells fixed before (the physicochemical damage model) and just after (the biological response model) irradiation...
Tue Aug 25, 2020 19:45
Abstract Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to acquire a high degree of focus points while still maintaining high throughput. Real‐time approaches decouple image acquisition from focusing, thus allowing for rapid scanning...
Tue Aug 25, 2020 19:24
ABSTRACT Wavefront‐shaping (WS) enables imaging through scattering tissues like bone, which is important for neuroscience and bone‐regeneration research. WS corrects for the optical aberrations at a given depth and field‐of‐view (FOV) within the sample; the extent of the validity of which is limited to a region known as the isoplanatic patch (IP). Knowing this parameter helps to estimate the number of corrections needed for WS imaging over a given FOV. In this paper, we first present direct transmissive...
Tue Aug 25, 2020 19:00
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